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KMID : 0880220090470050646
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Journal of Microbiology
2009 Volume.47 No. 5 p.646 ~ p.650
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Development of a Method Based on Surface Enhanced Laser Desorption and Ionization Time of Flight Mass Spectrometry for Rapid Identification of Klebsiella pneumoniae
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Xiao Daiwen
Yang Yongchang
Liu Hua
Yu Hua
Yan Yingjun
Huang Wenfang
Jiang Wei
Liao Weijin
Hu Qi
Huang Bo
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Abstract
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A method based on surface enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF MS) was developed for the rapid identification of Klebsiella pneumoniae by directly applying bacterial colonies without further protein extraction. A total of 40 K. pneumoniae and 114 other related microorganisms isolated clinically were analyzed by SELDI-TOF MS. An identification model for K. pneumoniae was established by artificial neural networks (ANNs) with classification accuracy of 100%. The model was blindly tested with 43 K. pneumoniae and 53 control bacteria again. The results showed that the model was successful with accuracy of 96.9%, sensitivity of 100% and specificity of 94.3%. This strategy is potential for rapid identification of K. pneumoniae.
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KEYWORD
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identification, K. pneumoniae, SELDI-TOF MS
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